I took this at the aquarium in Gatlingburg, Tn, and thought it came out pretty good so I decided I would post my settings for those of you that might be visiting an aquarium and need a starting point for the settings to use on your camera. I set my camera to aperture priority and set the aperture to F7.1 so I could get some depth of field. The ISO was set to 1600 with a shutter speed of 1/60 sec. I was using matrix metering and popped up the on board flash for fill light. This was shot with a Nikon D600 and Tamron 24-70 F2.8 lens. You do need to remember to watch out for the flash reflection in the glass.