Hey Loupey,
Finally got a chance to test out the 40D/300 f/4 IS combo with tubes for you. I tried all kinds of variables (IS on, IS off, Mode 1, Mode 2, handheld, tripod, etc.). Bottom line, I had NO focusing issues of any kind. The only thing I did notice is that it was hyper-sensitive to movement, and the focus would re-sync if it detected any.
Here are a few from the test: