Hey Loupey,
Finally got a chance to test out the 40D/300 f/4 IS combo with tubes for you. I tried all kinds of variables (IS on, IS off, Mode 1, Mode 2, handheld, tripod, etc.). Bottom line, I had NO focusing issues of any kind. The only thing I did notice is that it was hyper-sensitive to movement, and the focus would re-sync if it detected any.
Here are a few from the test:
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). The problem is that the IS causes the image to "jump" just as the shutter is tripped and I can see the jumped image sliding back into position just as the mirror flips back down. This "swipe" of the image during the exposure is what is causing the smeared image.
